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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Virology. 2015 Aug 21;485:492–501. doi: 10.1016/j.virol.2015.07.018

Fig.7.

Fig.7

Viral protein P3 was required to rescue replication of P23-3C(RBM). (−) Strand synthesis was assayed using PIRCs isolated from HeLa S10 reactions as described in Materials and methods. Reactions contained P23 RNA containing a 3C-RNA binding mutation (3C(K12N/R13N or 3C(RBM)) and a complementing RNA expressing the indicated protein. Labeled product RNA was analyzed as described in Fig.3 and quantitated as described in Fig.4.