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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Clin Immunol. 2016 Jan 28;164:85–94. doi: 10.1016/j.clim.2016.01.015

Figure 1. BAFFR-Fc binds BAFF specifically and inhibits splenocyte proliferation.

Figure 1

(A) BAFF ELISAs were performed with BAFFR-Fc (Vaccinex). Commercially available murine BAFFR-Fc was used as a positive control. Data represent an average of duplicate measurements and SD is shown. (B) Splenocytes were incubated with murine BAFF (0.5 μg/mL) in the presence of increasing concentrations of R&D BAFFR-Fc or Vaccinex BAFFR-Fc (0.02 - 1.0 μg/mL). The titration curve was fitted using four-parameter sigmoidal curve fit (R2 = 0.99). (C) Splenocytes were isolated from C57BL/6 females (8-10 wks). Proliferation was quantified using alamar blue incorporation. Data represent an average of triplicate measurements. Unstim = unstimulated. Significance was determined using Student's t-test. Mean and SEM are shown (**p < 0.01).