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. 2016 Mar 7;27(2):75–83. doi: 10.7171/jbt.16-2702-003

TABLE 2.

Assay Performance by Target Class and Assay Source

Performance metric All Long mRNA Long noncoding Short noncoding miRNA TaqMan Custom
IFC1 target 48 18 9 6 15 35 13
IFC1 targets detected 42 17 7 5 13 32 10
IFC2–11 targets 73 32 11 7 23 60 13
IFC2–11 targets detected 61 30 8 6 17 52 9
IFC1–11 rawCt minimum 2.50 2.50 2.50 2.50 2.50 2.50 2.50
IFC1–11 rawCt maximuma 37.91 29.47 37.91 36.63 37.56 37.56 37.91
IFC2–11 intra-array median CV 1.65% 1.65% 1.53% 1.76%
IFC1–11 interarray median CV 6.21% 6.51% 11.99% 6.12% 6.24% 6.13% 10.70%

CV, coefficient of variation.

a

Not including rawCt > 38.