Table S1.
Summary of predicted ORFs in the PFV1 genome
| ORF | Coordinates | Length, aa | TMDs | Predicted function | HHpred/CD hit | Blast hit | Identity (%), E-value |
| ORF1-196 | 317.0.754 | 145 | |||||
| ORF2-113 | 762.0.1103 | 113 | |||||
| ORF3-88 | 1106.0.1372 | 88 | |||||
| ORF4-52 | 1348.0.1506 | 52 | |||||
| ORF5-112 | 1484.0.1822 | 112 | |||||
| ORF6-93 | 1816.0.2097 | 93 | |||||
| ORF7-110 | 2094.0.2426 | 110 | |||||
| ORF8-120 | 2423.0.2785 | 120 | |||||
| ORF9-65 | 2786.0.2983 | 65 | TTV1 (CAA32969) | 21/64 (33), 3e-02 | |||
| ORF10-214 | 2976.0.3620 | 214 | 1 | N terminal similar to TTV1 (CAA32970); C terminal similar to TTV1 (CAA32971) | 25/84 (30), 2e-06; 20/53 (38), 9e-05 | ||
| ORF11-122 | 3617.0.3985 | 122 | TTV1 (CAA32973) | 19/79 (24), 2e-05 | |||
| ORF12-189 | 3966.0.4535 | 189 | Cas4-like nuclease | HHpred hit to Cas4 from Pyrobaculum calidifontis JCM 11548 (4r5q), P = 98.1; CD hit to Cas4 (TIGR00372), E = 2.4e-05 | TTV1_orf7-74 | 29/72 (40), 1e-08 | |
| ORF13-65 | 4537.0.4734 | 65 | |||||
| ORF14-63 | 4727.0.4918 | 63 | 2 | TTV1 (CAA32979) | 12/22 (55), 4e-04 | ||
| ORF15-67 | 4915.0.5118 | 67 | 3 | HHpred hit to DUF4536 (PF15055), P = 92 | |||
| ORF16-109 | 5122.0.5451 | 109 | |||||
| ORF17-155 | 5453.0.5920 | 155 | |||||
| ORF18-78 | 5917.0.6153 | 78 | |||||
| ORF19-139 | 6150.0.6569 | 139 | |||||
| ORF20-129 | 6640.0.7029 | 129 | Nucleoprotein VP1 | TTV1 (CAA32982) | 39/131 (30), 6e-04 | ||
| ORF21-145 | 7026.0.7463 | 145 | Nucleoprotein VP2 | A stretch of 14 Gly residues at the C terminus, also present in TTV1_orf12 (CAA32983) | |||
| ORF22-174 | 7465.0.7989 | 174 | |||||
| ORF23-230 | 7986.0.8678 | 230 | Trypsin-like Ser proteases | HHpred hits to various trypsin-like Ser proteases (PF13365), e.g., from Human astrovirus1 (2w5e), P = 96.7 | TTV1 (CAA32996); hyperthermophilic Archaeal virus 1 (YP_003773430) | 64/184(35); 9e-22; 57/184 (31); 6e-11 | |
| ORF24-166 | 8709.0.9209 | 166 | 2 | Sheath protein VP3 | TTV1_TP3 (CAA32989) | 42/152 (28), 2e-07 | |
| ORF25-125 | 9212.0.9589 | 125 | |||||
| ORF26-242 | Complement (9700.0.10428) | 242 | Glycosyltransferase (GT-A fold; cd02510) | HHpred hit to various Gtases (cd02510), P = 97.4 | TTV1 (CAA32992); Los Azufres archaeal virus 1 (AHA92064) | 67/215 (31); 7e-09; 33/92(36); 1e-04 | |
| ORF27-471 | 10402.0.11889 | 495 | |||||
| ORF28-481 | 11918.0.13363 | 481 | O-glycoside hydrolase (5-bladed β-propeller fold; family 43) | N-terminal region (amino acids 56–150): HHpred hit to a noncatalytic jelly-roll Lyase_N domain (PF09092) required for binding of the protein to long glycosaminoglycan chains, P = 94. C-terminal region (amino acids 111–400): HHpred hits to various family 43 O-glycoside hydrolases (PF04616), e.g., from Bacteroides thetaiotaomicron (3qz4), P = 95.5. | Microvirga lupini (WP_036352061) | 75/268(28); 2e-07 | |
| ORF29-143 | 13374.0.13805 | 143 | 1 | Carbohydrate-binding protein (C-type lectin-like fold; PF02369) | HHpred hit to integrin-binding protein (1cwv), P = 95.4 | ||
| ORF30-100 | 13847.0.14149 | 100 | 1 | Carbohydrate-binding protein (C-type lectin-like fold) | HHpred hit to integrin-binding protein (1cwv), P = 99.2 | Candidatus Methanoperedens nitroreducens (KCZ71275) | 39/81(48); 2e-07 |
| ORF31-40 | 14176.0.14298 | 40 | 1 | ||||
| ORF32-68 | 14323.0.14529 | 68 | |||||
| ORF33-343 | 14526.0.15557 | 343 | 2 | ||||
| ORF34-119 | 15587.0.15925 | 112 | 3 | ||||
| ORF35-96 | 15925.0.16215 | 96 | 3 | ||||
| ORF36-135 | 16203.0.16538 | 111 | |||||
| ORF37-87 | 16568.0.16831 | 87 | Ferritin (cd01045) | Numerous hits to ferritins from various archaea and bacteria, e.g., 3k6c (P = 99.8), 2e0z (P = 99.8), 1vjx (P = 99.3) | Pyrococcus furiosus DSM 3638 (NP_578921); Thermoproteus tenax Kra 1 (YP_004892222) | 30/83 (36), 9e-10; 32/79 (41), 2e-06 | |
| ORF38-71 | 16848.0.17063 | 71 | |||||
| ORF39-171 | 17047.0.17550 | 167 | Carbohydrate-binding protein (lectin) | HHpred hits to eukaryotic carbohydrate-binding lectins from Polyporus squamosus (3phz, P = 99.9) and Marasmius oreades (3ef2, P = 99.5) | Candidatus Nanosalina sp. J07AB43 (EGQ44217); Environmental halophage eHP-11 (AFH21856) | 33/107 (31), 1e-09; 33/99 (33), 6e-08 |
PFV1 gene products that share significant similarity (BLASTP cutoff of E = 1e-05) with sequences in the nonredundant protein database are indicated by boldface type. TMD, transmembrane domain.