Fig. 1.

HCV purification flow chart. Schematic description of the steps used to purify extracellular HCV virions. (A) Con1/Jc1 virus produced in SFM was affinity purified using HCV-neutralizing antibodies prior to proteomics analysis. (B) Tag-clone 2 produced in FBS-containing media was sedimented over a buoyant density gradient and affinity purified with His-Dynabeads. Imidazole-eluted material was subjected to MS. hpi, hours postinfection; qPCR, quantitative PCR; TCID₅₀, 50% tissue culture infectious dose; WB, Western blot.