Fig. S4.

RNAi of candidate HCV-associated host factors. (A) RNAi in HCV-reporter cells. A Huh-7.5–derived clone encoding the HCV tagRFP-nls-IPS cellular reporter system was transfected with scrambled (a), HCV (b), Nup98 (c), and PTGFRN (d) siRNAs. Silenced cells were infected 2 d later with Con1/Jc1 at low MOI (0.05). Representative images were taken 3 d postinfection (dpi). Uninfected cells display a mitochondrial fluorescent pattern, whereas RFP nuclear translocation upon NS3/4a-mediated cleavage of IPS1 is a signature of productive HCV infection. (Scale bars, 100 μm.) (B) Viability of siRNA-transfected cells at the time of harvesting, as measured by FACS. (C) Percentage of down-regulation of target mRNA levels after siRNA transfection compared with control cells, as measured by RT-quantitative PCR. Values were normalized to GAPDH RNA levels.