Table S1.
Recovery of HCV infectivity and viral RNA throughout the purification protocol
| Sample | Total IU | IU, % | Total RNA | RNA, % | 1/SI |
| Wild-type–HCV | |||||
| Stock | 1.9E + 08 | 100.0 | 3.4E + 12 | 100.0 | 1.8E +04 |
| Concentrated | 1.6E + 08 | 85.3 | 2.8E + 12 | 81.7 | 1.8E +04 |
| FT concentr | 0.0E + 00 | 0.0 | 3.2E + 10 | 1.0 | — |
| HP input | 1.6E + 08 | 100.0 | 2.8E + 12 | 100.0 | 1.8E + 04 |
| HP FT | 1.1E + 07 | 6.8 | 1.4E + 11 | 5.2 | 1.3E + 04 |
| W1 | 1.6E + 06 | 1.0 | 7.5E + 10 | 2.7 | 4.7E + 04 |
| W2 | 2.3E + 05 | 0.1 | 5.9E + 09 | 0.2 | 2.6E + 04 |
| HP eluted | 1.3E + 08 | 84.8 | 1.4E +12 | 49.6 | 1.0E + 04 |
| Dialyzed post-HP | 1.6E + 11 | 99,683.5 | 1.2E + 12 | 44.4 | 7.9E + 00 |
| Tag-HCV | |||||
| Stock | 7.5E + 07 | 100.0 | 3.6E + 12 | 100.0 | 6.6E + 04 |
| Concentrated | 8.4E + 07 | 111.4 | 2.9E + 12 | 82.4 | 3.5E + 04 |
| FT concentr | 0.0E + 00 | 0.0 | 4.0E + 10 | 1.1 | — |
| HP input | 1.3E + 08 | 100.0 | 2.4E + 12 | 100.0 | 1.8E + 04 |
| HP FT | 2.8E + 06 | 2.1 | 1.3E + 11 | 5.3 | 4.6E + 04 |
| W1 | 1.6E + 06 | 1.2 | 4.9E + 10 | 2.0 | 3.1E + 04 |
| W2 | 1.6E + 05 | 0.1 | 2.0E + 10 | 0.8 | 1.2E + 05 |
| HP eluted | 1.2E + 08 | 90.7 | 1.3E + 12 | 52.4 | 1.1E + 04 |
| Dialyzed post-HP | 1.2E + 11 | 89738.8 | 9.5E + 11 | 38.8 | 7.9E + 00 |
FT concentr, flow-through concentration; HP, heparin; 1/SI, 1/specific infectivity; IU, infectious unit(s); W1, wash 1; W2, wash 2.