Table S1.
Peptide sequences of predicted calmodulin binding sites
| Peptide | Amino acid position | Amino acid sequence |
| P1 | 788–810 | LTCSRWKKVQWCSLSVIKLKNKI |
| P2 | 812–834 | YRAEACIKMQKTIRMWLCKRRHK |
| P3 | 832–856 | RHKPRIDGLVKVGTLKKRLDKFNEV |
| P4 | 855–877 | EVVSVLKDGKPEMNKQIKNLEIS |
| P5 | 872–895 | KNLEISIDTLMAKIKSTMMTQEQI |
| P6 | 889–912 | MMTQEQIQKEYDALVKSSEELLSA |
| P7 | 925–944 | RLRRIQEEMEKERKRREEDE |
| P8 | 950–974 | EEEERRMKLEMEAKRKQEEEERKKR |
| P9 | 975–944 | EDDEKRIQAEVEAQLARQKE |
| P10 | 1,016–1,036 | IAQSEAELISDEAQADLALRR |
| P2–3 | 812–860 | YRAEACIKMQKTIRMWLCKRRHKPRIDGLVKVGTLKKRLDKFNEVVSVL |
| P2–3‡ | 812–858 | YRAEACIKMQKTIRMWLCKRRHKPRIDGLVKVGTLKKRLDKFNEVVS |
| P2–1/23 | 812–847 | YRAEACIKMQKTIRMWLCKRRHKPRIDGLVKVGTLK |
| P1/22–3 | 825–860 | RMWLCKRRHKPRIDGLVKVGTLKKRLDKFNEVVSVL |
| HB1 | 833–874 | HKPRIDGLVKVGTLKKRLDKFNEVVSVLKDGKPEMNKQIKNL |
| HB1‡ | 833–874 | HKPRIDGLVKVGTLKKRLDKGNEGGSAGKDGKPEMNKQIKNL |
The peptides of potential calmodulin binding sites, as determined for human myosin-VI, were used in the tryptophan fluorescence assays to measure the calmodulin binding affinity. The numbers specify the amino acid position in the human myosin-VI sequence.