Fig. S3.

IL7 secretion diminishes in both mouse and human embryo after fertilization. (A) IL7 protein levels in spent media during oocyte maturation and embryo culture in mouse. COCs were incubated in maturing media for 20 h, and culture supernatant was collected for IL7 ELISA. After in vitro fertilization, the spent embryo media were obtained when the embryos reached to two-cell and blastocyst stages to assess secreted IL7 with ELISA. IL7 levels were normalized to the number of oocytes/embryos incubated. Error bars correspond to the SEM, and data are from at least three experiments from biologically different samples. *P < 0.05 vs. two-cell and blastocyst stages. (B) IL7 protein levels in spent media during COC and embryo culture in human. After oocyte retrieval, COCs were washed and incubated 3 h before cumulus cell stripping for intracytoplasmic sperm injection, and spent media were collected for IL7 ELISA. After fertilization check (day 1), the embryos were cultured to blastocyst stage (day 5). The human embryo media were obtained when the embryos reached to cleavage (day 3) and blastocyst (day 5) stages to assess secreted IL7 with ELISA. IL7 levels were normalized to the number of oocytes/embryos incubated and incubation time. Error bars correspond to the SEM, and data are from at least three experiments from biologically different samples. ^#P < 0.05 vs. cleavage and blastocyst stages, ANOVA.