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. 2016 Feb 16;113(9):2502–2507. doi: 10.1073/pnas.1520040113

Fig. 2.

Fig. 2.

(A) General map of the landing pad sequence inserted into the various strains. The green region represents a target sequence for allelic replacement by a set of five oligos shown in the targeting box. Degenerate bases are shown for each oligo in red. The cat gene conferring resistance to chloramphenicol was included in the landing pad to allow for cos-MAGE to be performed (see SI Materials and Methods for details). Allelic replacement frequency of the various test oligonucleotides targeting the tetR landing pad sequence in (B) E. coli K-12 MG1655, (C) TS616 derivative of S. enterica serovar Typhimurium LT2, and (D) C. freundii (strain ATCC8090). The values are the means of two independent measurements using Illumina deep-sequencing; error bars represent SEMs. An asterisk denotes oligos generating the same mismatch as another oligo to demonstrate context dependency of allelic replacement. DmutS denotes deletion of the mutS gene.