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Effects of coselection on allele replacement efficiency. (A) E. coli K-12 MG1655(pORTMAGE) allelic replacement frequency with and without coselection. (B) S. enterica TS616(pORTMAGE) allelic replacement frequency with and without coselection. (C) C. freundii ATCC8090(pORTMAGE) allelic replacement frequency with and without coselection. The values are the means of two independent measurements using Illumina deep-sequencing, error bars represent SEMs.
