Fig. S7.

Sequence randomization of 6 nucleotides of the asnA gene of (A) E. coli K-12 MG1655, (B) TS616 derivative of S. enterica serovar Typhimurium LT2, and (C) C. freundii (strain ATCC8090). A comparison is shown using the control pSIM8 (first stacked column for each position) versus pORTMAGE (indicated with “+pORTMAGE” for the given stacked column). The frequencies of each nucleotide at each of the mutated genomic positions are represented in each stacked column. The genomic positions of each randomized nucleotide relative to the first nucleotide of the gene, as well as the wild-type allele (shown in parentheses) are indicated.