Fig. 6.

JNK signaling is activated and required in the FCs during developmental PCD of the NCs. (A–D’) puc-lacZ/TM3 was used as a reporter for JNK activity (arrows). Stage 11–14 egg chambers are stained with α–β-Gal (red) and DAPI (cyan). puc-lacZ is initially detected in FC nuclei during stage 11 (A and A’) and becomes most highly expressed in stages 12 and 13 (B and B’ and C and C’). puc-lacZ is also apparent in some FCs along the DA in stage 14 (D and D’). (Scale bar, 50 μm.) These data are quantified in Fig. S5A. (E–H) Persisting nuclei (arrows) are observed in stage 14 egg chambers of JNK pathway knockdowns stained with DAPI (blue). (E) GR1-GAL4/UAS-luciferase^RNAi control does not have persisting NC nuclei. (F–H) Persisting NC nuclei are found in (F) GR1-GAL4/UAS-kayak^RNAi, (G) GR1-GAL4/UAS-jra^RNAi, and (H) GR1-GAL4/UAS-Cka^RNAi. (Scale bar, 20 μm.) (I) Quantification of persisting nuclei in JNK pathway knockdowns, including bsk^DN, kayak^RNAi, jra^RNAi, and Cka^RNAi (data from two distinct Cka RNAi lines were combined), compared with the control GR1-GAL4/UAS-luciferase^RNAi. Data presented are mean ± SEM. ****P ≤ 0.0001.