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. 2016 Feb 16;113(9):E1246–E1255. doi: 10.1073/pnas.1522830113

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Fig. S2. — Ced-12 knockdowns and draper^∆5 mutants have reduced fecundity and defects in border cell migration. (A) The knockdown of Ced-12 in all FCs results in sterility. Ced-12 single knockdown (UAS-GAL4/CyO; GR1-GAL4 G89/UAS-Ced-12^RNAi) and draper^RNAi Ced-12^RNAi double knockdowns (UAS-GAL4/UAS-draper^RNAi; GR1-GAL4 G89/UAS-Ced-12^RNAi) do not produce any progeny. The control (UAS-GAL4/UAS-draper^RNAi; TM6B/UAS-Ced-12^RNAi) and draper single knockdown (UAS-GAL4/UAS-draper^RNAi; GR1-GAL4 G89/MKRS) produce similar numbers of progeny. These data represent progeny produced over the entire lifetime of the females tested. n, number of females. Data presented are average pupae per female per day ± SEM. ****P < 0.0001. (B–D) Border cell migration is defective when Ced-12 is knocked down in all FCs. (B) Ced-12 knockdowns (GR1-GAL4 UAS-mCD8-GFP/UAS-Ced-12^RNAi) have impaired border cell migration compared with control (GR1-GAL4 UAS-mCD8-GFP/TM3). Border cell migration in draper knockdown (UAS-draper^RNAi/+; GR1-GAL4/+) is normal. Data presented are mean % migration ± SEM. ****P ≤ 0.0001. (C and D) Egg chambers express membrane GFP in the FCs (green), including the border cells (arrows). (Scale bar, 50 µm.) (C) Border cells in GR1-GAL4 UAS-mCD8-GFP/TM3 (control) stage 10 egg chambers successfully migrate to the NC/oocyte interface. (D) GR1-GAL4 UAS-mCD8-GFP/UAS-Ced-12^RNAi egg chambers have impaired border cell migration. (E–G) Border cell migration is impaired in draper^∆5 mutants. (E and F) Egg chambers stained with anti-FasIII (red) to mark polar cells within the border cell cluster (arrows). (E) w¹¹¹⁸ control stage 10 egg chamber has normal border cell migration. (F) Border cell migration is delayed in draper^∆5 mutant. (G) Quantification of border cell migration. Data presented are mean % migration ± SEM. ****P ≤ 0.0001. (H) Border cell migration is not delayed when Ced-12 is knocked down only in the stretch FCs. (I) draper^RNAi (PG150-GAL4/+; UAS-draper^RNAi/+) and Ced-12^RNAi (PG150-GAL4/+; UAS-Ced-12^RNAi/+) single knockdowns have reduced egg laying compared with the control (PG150-GAL4/+; UAS-luciferase^RNAi/+). Egg laying was quantified in females 21–25 d old. n, number of females. Data presented are average number of eggs laid per female per day ± SEM. ***P ≤ 0.001 or **P ≤ 0.01.