Fig. S3.

Intracellular trafficking genes are required in the FCs for NC removal and act in the same pathway as draper. (A) Quantification of persisting nuclei in Rab5, Rab7, and Rab35 FC knockdowns identified in the candidate screen. The quantification of GR1-GAL4/UAS-luciferase^RNAi was used as the control for multiple experiments and was first presented in Fig. 1Q. Data presented are mean ± SEM. ****P ≤ 0.0001. (B and C) draper and Rab35 act in the same pathway to promote NC removal. (B) Quantification of persisting NC nuclei in draper^∆5 Rab35^RNAi double knockdowns. draper^∆5 Rab35^RNAi double knockdown (purple, draper^∆5 UAS-Rab35^RNAi/draper^∆5 GR1-GAL4) has a similar persisting NC nuclei phenotype to draper^∆5 (red, draper^∆5 UAS-Rab35^RNAi/draper^∆5) but is significantly weaker than Rab35^RNAi (blue, draper^∆5 UAS-Rab35^RNAi/GR1-GAL4 UAS-mCD8-GFP). Mixed sibling controls lack either the GAL4 driver or the RNAi constructs. Data presented are mean ± SEM. ****P ≤ 0.0001. (C) Alternative quantification of data presented in Fig. S3B. The number of persisting nuclei (PN) per stage 14 egg chamber was categorized into bins of 0 PN, 1–3 PN, 4–6 PN, 7–9 PN, 10–12 PN, and 13–15 PN, and the percentage of stage 14 egg chambers in each bin was calculated.