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. 2016 Feb 16;113(9):E1316–E1325. doi: 10.1073/pnas.1525466113

Fig. 4.

Fig. 4.

Microglial phagocytosis is impaired in Rag-5xfAD mice. (A and F) Representative immunohistochemical images of microglia (Iba1), Aβ (82E1), and microglial phagolysosomes (CD68). (B–E and G–J) Three-dimensional reconstruction of microglia (blue; Iba1), Aβ (red; 82E1), phagolysosomes inside microglia (green; colocalization of CD68 and Iba1), and amyloid within phagolysosomes (white; colocalization of 82E1 and microglial phagolysosomes). (K) Calculation of the Aβ internalization ratio (Aβ within phagolysosomes, normalized to microglia number and total Aβ within the field) revealed a 39.6% decrease in Aβ internalization in Rag-5xfAD microglia compared with WT-5xfAD. Data are represented as mean ± SEM. Student’s t test, P < 0.05; n > 8 animals/genotype.