Fig. S1.

Breeding paradigm and immune phenotype confirmation of the Rag-5xfAD mouse model. (A) Abbreviated breeding diagram showing the strategy used to generate the Rag-5xfAD mouse model and controls. (B–E) Flow cytometry performed on splenocytes of 6-mo-old animals confirmed that all immune-incompetent animals lacked B, T, and NK cells regardless of AD transgene status. Analysis of immune-competent animals revealed that percentages of these cell populations were not significantly different between WT-WT and WT-5xfAD animals except in the case of CD4 T cells (B), which were significantly decreased in WT-5xfAD mice vs. WT-WT mice. n ≥ 6 animals/group and *P < 0.05.