Fig. S3.

Evans Blue analysis demonstrates no detectable changes in general BBB permeability. (A) Representative pictures of the liver, kidney, and brain of WT-WT, WT-5xfAD, Rag-WT, Rag-5xfAD, and uninjected control mice after killing and perfusion. (B) Spectroscopy analysis for levels of Evans Blue was used to measure changes in permeability according to previously published protocols (33). Analysis revealed no detectable levels of Evans Blue in the brains of any of the four genotypes and significant levels in both peripheral tissues examined. Absorbance values of uninjected control tissue was subtracted from the recorded absorbance of all tissue from all four genotypes. Data represented as mean ± SEM; n = 3 animals/genotype.