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Protein expression and secretion by B. pertussis strains. (A and B) Immunoblot analysis of cell pellet and culture supernatant fractions from the indicated strains prepared from midlog or stationary phase cultures in Stainer–Scholte medium. Blots were probed with antisera specific for FhaB, CyaA, Prn, Bsp22, or pertussis toxin subunit S4.
