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. 2016 Feb 8;113(9):2388–2393. doi: 10.1073/pnas.1600375113

Fig. 4.

Fig. 4.

Genetically encoded biosensors were used to identify optimized 3HP production conditions. (A) Eight concentrations of cerulenin and four concentrations of IPTG were combinatorially evaluated in triplicate. Growth-normalized fluorescence of the 96 samples was measured after 24 h (darker shades indicate higher fluorescence). We found that 20 μg/mL cerulenin combined with 1,000 μM IPTG resulted in the greatest biosensor response. (B) The 3HP titer was determined by LC/MS for a range of IPTG concentrations while cerulenin was held constant at 20 μg/mL (C) The 3HP titer was determined by LC/MS for a range of cerulenin concentrations while IPTG was held constant at 1,000 μM. In both cases fluorescence and titer trended together, verifying the optimal concentrations of IPTG and cerulenin determined by fluorescence alone. Error bars represent the 95% confidence interval (n = 3).