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Cells without the acuR-based 3HP biosensor helper plasmids do not produce fluorescence in the presence of 3HP. The fluorescent response to the addition of acrylate indicates that the biosensor is functional, but insulated against activation by 5 mM 3HP without the enzymes necessary to convert 3HP to acrylate. The confidence band represents the 95% confidence interval determined by the SEM (n = 3).
