Fig. S3.

Formation of 3HP is observed in real time. (A) The prpR-based 3HP biosensor reports 3HP production progress in real time. Addition of cerulenin increases the malonyl-CoA pool, providing a boost in 3HP production (purple line). Addition of IPTG increases mcr expression and further increases 3HP production (blue line). Cerulenin and IPTG have no impact on the fluorescent response of cells without mcr (gray and black lines). (B) Fluorescence of the prpR-based biosensor after 12 h. Error bars and confidence bands represent the 95% confidence interval (n = 3).