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. 2016 Feb 16;113(9):E1226–E1235. doi: 10.1073/pnas.1600813113

Fig. 1.

Fig. 1.

MEKK2 promotes osteoblast activity in vivo and in vitro. (A and B) Immunohistochemistry for MEKK2 (A) and phospho-MEKK2/3 (B) at 40× and 100× magnification, respectively on 1-wk-old mouse tibias. (C) Quantification of BV/TV and cortical thickness (C.Th) in the femur of 4-wk-old Mekk2+/+ and Mekk2−/− mice. All error bars indicate SEM. P < 0.05 was considered statistically significant by two-tailed, unpaired Student’s t test. *P < 0.05 and **P < 0.01; n = 5 mice per group. (D) 3D reconstructions of calvaria of 4-wk-old Mekk2+/+ and Mekk2−/− mice. (E) Histomorphometric analysis of tibias from 8-wk-old Mekk2+/+ and Mekk2−/− mice; n = 5 mice per group. (Upper) Photomicrographs of dual-labeled trabecular bones. (Lower) Quantification of BFR and MAR. *P < 0.05. (F) Serum levels of P1NP and CTX in 8-wk-old Mekk2+/+ and Mekk2−/− mice; n = 5 mice per group. *P < 0.05; N.S, not significant. (G) In situ hybridization for Ocn on tibias from 1-wk-old Mekk2+/+ and Mekk2−/− mice. (Magnification: 40×.) (H and I) Primary calvarial osteoblasts were isolated and placed under differentiation conditions for 21 d. (H) Von Kossa staining was performed to determine osteoblast mineralization activity. (I) After 7 d of culture, the expression of osteoblast marker genes was analyzed by RT-PCR. **P < 0.01; ***P < 0.001.