Table 3.
Serum cytokines and chemokines: Serum cytokine concentrations 1 and 7 days following instillation 200 μl of citrate vehicle 200 μg 20 nm AgNP or 1 day post silver acetate (AgAc) instillation. Statistical significance p<0.05 compared to citrate vehicle b determined by student’s t-test. n=4–5. Data reported as mean ± SEM.
| Cytokine/Chemokine (pg/ml) | Citrate | AgNP | AgAc | ||
|---|---|---|---|---|---|
| 1 day | 7 days | 1 day | 7 days | 1 day | |
| G-CSF | 11.1 ± 3.93 | 53.0 ± 38.1 | 90.6 ± 21.1b,c | 119.0±14.5 | 0.0 ± 0.0 |
| GM-CSF | 0.0 ± 0.0 | 10.9 ± 10.9 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| MIP-1α | 20.9 ± 3.7c | 28.2 ± 8.9 | 41.5 ± 1.53b,c | 40.4 ± 1.49 | 0.0 ± 0.0 |
| IL-2 | 110.0 ± 29.3c | 175.0 ± 65.3 | 312.0 ± 10.1b,c | 328 ± 23.3b | 0.0 ± 0.0 |
| IL-13 | 0.0 ± 0.0 | 20.7 ± 20.7 | 422.0 ± 155.0b,c | 792 ± 224b | 0.0 ± 0.0 |
| IL-10 | 23.9 ± 8.1c | 51.3 ± 31.2 | 101.0 ± 5.4b,c | 99.8 ± 9.51 | 0.0 ± 0.0 |
| INFγ | 0.0 ± 0.0 | 187.0±187.0 | 126.0 ± 126.0 | 305.0 ± 39.6 | 0.0 ± 0.0 |
| IL-5 | 243 ± 43.6 | 351.0 ± 79.6 | 331.0 ± 7.8 | 324.0 ± 7.82 | 8.74 ± 8.74 |
| IL-17α | 54.6 ± 18.3c | 94.7 ± 37.9 | 155.0 ± 7.8b,c | 167 ± 8.32 | 0.0 ± 0.0 |
| RANTES | 721.0 ± 26.8c | 663.0 ± 130.0 | 1138.0 ± 52.1b,c | 924.0 ± 60.4 | 143 ± 13.7 |
Note: Serum cytokine concentrations 1 day and 7 days following 200μl instillation of Citrate vehicle or 200 μg 20 nm AgNP or silver acetate (AgAc). Statistical significance p<0.05 compared to
b
Citrate vehicle at matched time-point or compaired to
c
AgAc determined by one-way ANOVA and Tukey’s post-hoc test for multiple compairisions. Data displayed as mean ± SEM, n=4–5.