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. 2016 Mar 2;11:823–835. doi: 10.2147/IJN.S101846

Figure 4.

Figure 4

Activation status of untreated and LPS-activated BM-DCs in 2D culture dish and 3D hybrid NFS.

Notes: (A) Expression levels of CD86 on the surface of PKH67-labeled BM-DCs cultured with or without LPS for 24 hours in 2D culture dish and 3D hybrid NFS. (B) Confocal images of untreated and LPS-activated BM-DCs in 2D culture dish and 3D hybrid NFS using FITC-conjugated phalloidin and PE-conjugated antibody against MHC Class II. (C) Patterns of actin and phosphorylated FAK distribution in activated BM-DCs in 2D culture dish and 3D hybrid NFS. Data are representative of three independent experiments.

Abbreviations: NFS, nanofibrous scaffold; BM-DCs, bone marrow-derived dendritic cells; LPS, lipopolysaccharide; MHC, major histocompatibility complex; FAK, focal adhesion kinase; SEM, scanning electron microscope; 2D, two dimensional; 3D, three dimensional; FITC, fluorescein isothiocyanate; PE, phycoerythrin.