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. 2016 Mar 2;11:823–835. doi: 10.2147/IJN.S101846

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© 2016 Kim et al. This work is published and licensed by Dove Medical Press Limited

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In vitro culture of total cells isolated from colon cancer tissue in 2D culture dish and 3D hybrid NFS.

Notes: (A) Confocal analysis of tumor tissue from colon cancer-bearing mice using FITC-conjugated anti-reticular fiber antibody, PE-conjugated anti-CD11c antibody, and DAPI. (B) Culture of total cells isolated from tumor tissue of colon cancer-bearing mice in 2D culture dish and 3D hybrid NFS for the indicated times. Data are representative of three independent experiments.

Abbreviations: NFS, nanofibrous scaffold; DAPI, 4′,6-diamino-2-phenylindole; 2D, two dimensional; 3D, three dimensional; FITC, fluorescein isothiocyanate; DIC, differential interference contrast; PE, phycoerythrin.