Abstract
An automated micromethod for measuring iron concentration in serum employing thioglycollic acid and bathophenantroline sulphonate as reducing agent and chromogen respectively, is described. Measurements are carried out using an AutoAnalyzer (Technicon) and require 0·1 ml of sample. The reproducibility, assessed by the mean coefficient of variation (1·9%), and the mean recovery of iron added to samples (99·5%), as well as the correlation between the serum iron values estimated by the described method and the method recommended by the Expert Panel on Iron of the International Committee for Standardization in Haematology (r = 0·9779; p < 0·001; y = 0·9713 x −0·0546), is highly satisfactory. The method can also be used for measuring total ironbinding capacity of serum. The method appears equally suitable for routine work and research studies when a large number of samples and a small volume of serum are available.
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Selected References
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- Fielding J., Ryall R. The action of reducing agents on the dissociation of iron transferrin with special reference to thioglycollate. Clin Chim Acta. 1970 Jun;28(3):423–429. doi: 10.1016/0009-8981(70)90068-9. [DOI] [PubMed] [Google Scholar]
- Giovanniello T. J., DiBenedetto G., Palmer D. W., Peters T., Jr Fully and semi-automated methods for the determination of serum iron and total iron-binding capacity. J Lab Clin Med. 1968 May;71(5):874–883. [PubMed] [Google Scholar]
- Kauppinen V., Gref C. G. Automated determination of serum iron. Scand J Clin Lab Invest. 1967;20(1):24–28. doi: 10.1080/00365516709076915. [DOI] [PubMed] [Google Scholar]
- Klein B., Kleinman N., Searcy R. L. Application of Fe+2-5-pyridyl-benzodiazepin-2-ones to the automated determinations of serum iron and iron-binding capacity. Clin Chem. 1970 Jun;16(6):495–499. [PubMed] [Google Scholar]
- RAMSAY W. N. The determination of the total iron-binding capacity of serum. Clin Chim Acta. 1957 Jun;2(3):221–226. doi: 10.1016/0009-8981(57)90106-7. [DOI] [PubMed] [Google Scholar]
- Yee H. Y., Zin A. An autoanalyzer procedure for serum iron and total iron-binding capacity, with use of ferrozine. Clin Chem. 1971 Sep;17(9):950–953. [PubMed] [Google Scholar]
- ZAK B., EPSTEIN E. AUTOMATED DETERMINATION OF SERUM IRON. Clin Chem. 1965 Jun;11:641–644. [PubMed] [Google Scholar]