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. 2016 Mar 7;11(3):e0150961. doi: 10.1371/journal.pone.0150961

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© 2016 Ren et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A) Western blot analyzed HBx and SIRT3 in cells transfected with indicated plasmids. β-actin was used as a loading control. (B-C) ROS levels in Huh-7 cells transfected with indicated plasmids were examined by carboxy-H2DCFDA fluorescence (B) or flow cytometry (C). Magnification, ×100. *, p<0.01. (D)The expression of HBx and SIRT3 in cells transfected with indicated plasmids and siRNA. β-actin was used as a loading control. (E-F) ROS levels in Huh-7 cells transfected with indicated plasmids and siRNA were examined by carboxy-H2DCFDA fluorescence (E) or flow cytometry (F). Magnification, ×100. *, p<0.05. (G) The GSH/GSSG ratio in Huh-7 cells treated with 1 mM H₂O₂ or transfected with indicated plasmids was analyzed by GSH/GSSG-Glo™ Assay. *, p<0.01.