Cell Culture Vessel	Surface Area (cm2)	Number of Cells Seeded for Routine Maintenance	Number of Cells Seeded for Sensory Epithelia Differentiation	Number of Cells Seeded for Neuronal Differentiation	Scaling Factor Relative to  60 mm Dish	Volume of Media Used (ml)
Multwell Plates
96	0.3	10,000	20,000	10,000-50,000	0.015	0.1
24	2	90,000	180,000	100,000-150,000	0.100	0.5
12	4	180,000	360,000	300,000	0.200	1
6	10	500,000	1,000,000	500,000-750,000	0.500	2
Dishes
35 mm	10	500,000	1,000,000	500,000	0.500	2
60 mm	20	1,000,000	2,000,000	1,000,000-1,500,000	1.000	3
100 mm	60	2,500,000	5,000,000	2,500,000- 3,000,000	3.000	8