Step 1 – Adjust the input
Adjusted input = Raw Ct(input) - Log2(fraction of total chromatin)
	Raw Ct	input from total chromatin	Log210
Input	29.5	10%	3.32
Adjusted input = 29.5 – 3.32 = 26.18
Step 2 - % of input calculations
Sample	Raw Ct	Ct(adjusted input) –Ct(sample)	2 Ct(adjusted input) –Ct(sample) *100%
Input	26.18
ChIP-Ab	31.45	-5.27	2.59
No-Ab	34.5	-8.32	0.31