Figure 2.

RNMT T77 Is Phosphorylated during G2/M Phase

(A) HeLa cells expressing HA-RNMT were synchronized by double thymidine or thymidine-nocodazole block and released as indicated. In these cells, asynchronous cells (A) and asynchronous cells expressing HA-RNMT T77A (77A), WBs were performed to detect pT77 and total RNMT in HA-RNMT immunoprecipitates and cell cycle markers indicated in cell extracts. Above the blots, a chart depicts the average pT77/RNMT signal and SD for three independent experiments, calculated from WB using ImageJ software. Flow cytometry cell cycle analysis and phase percentage are reported below the blots.

(B) HeLa cells were synchronized, and endogenous pT77 and total RNMT were detected in RNMT immunoprecipitates.