Targeted region	Angle	Position of the electrode	Age of embryo	DNA amount/ concentration	Voltage	Size of electrode
Retrosplenial Cortex	330°- 0°	Center of the electrodes just anterior of the ear primordia	E14 (E11-E17)	1.5- 2 µl/ 2.7- 2 µg/µl	36- 38	3- 5 mm
Motor Cortex	0°- 40°	Center of the electrodes just anterior of the ear primordia	E14 (E11-E17)	1.5- 2 µl/ 2.7- 2 µg/µl	36- 38	3- 5 mm
Somatosensory Cortex	40°- 95°	Center of the electrodes just anterior of the ear primordia	E14 (E11-E17)	1.5- 2 µl/ 2.7- 2 µg/µl	36- 38	3- 5 mm
Piriform Cortex	95°- 110°	Center of the electrodes just anterior of the ear primordia	E14 (E11-E17)	1.5- 2 µl/ 2.7- 2 µg/µl	36-3 8	3- 5 mm
Cornu Ammonis (CA)1	250°- 275°	Center of the positive pole at the middle of the ear primordium	(E14-) E15	2 µl/ 2 µg/µl	38- 40	3- 5 mm
CA2	230°- 250°	Center of the positive pole at the middle of the ear primordium	(E14-) E15	2 µl/ 2 µg/µl	38- 40	3- 5 mm
CA3	210°- 230°	Center of the positive pole at the middle of the ear primordium	(E14-) E15	2 µl/ 2 µg/µl	38- 40	3- 5 mm
Dentate Gyrus	190°- 210°	Center of the positive pole at the middle of the ear primordium	(E14-) E15	2 µl/ 2 µg/µl	38- 40	3- 5 mm
Striatum	170°- 240°	Center of the electrodes at the level of the ear primordia	E12	1 µl/ 4 µg/µl	33	0.5 mm
Lateral Septal Nucleus	100°- 170°	Center of the electrodes at the level of the ear primordia	E12	1 µl/ 4 µg/µl	33	0.5 mm
Thalamus	25°- 40°	Center of the electodes just posterior the ear primordia	E12- E13	1-1.5 µl/ 4-2.7 µg/µl	33- 35	0.5- 3 mm
Hypothalamus	90°- 180°	Center of the electodes just posterior the ear primordia	E12- E13	1-1.5 µl/ 4-2.7 µg/µl	33- 35	0.5- 3 mm