Ingredient	Volume per Reaction (μl) (2)	Final Concentration	Volume per Master Mix (μl) (3)
2x LightCycler 480 Probes Master Mix	10	Proprietary	1050
ROX reference dye (4)	0.4	0.5 mM	42
PCR grade water	0.3		31.5
10 μM NorGIIF	1	500 nM	105
10 μM NorGIIR	1.8	900 nM	189
10 μM NorGIIP	0.5	250 nM	52.5
Total	14		1470