Ingredient	Volume per Reaction (μl) (2)	Final Concentration	Volume per Master Mix (μl)(3)
2x LightCycler 480 Probes Master Mix	10	Proprietary	1050
ROX reference dye (4)	0.4	0.5 mM	42
PCR grade water	1.4		147
10 μM HepF	1	500 nM	105
10 μM HepR	1	500 nM	105
10 μM HepP	0.2	100 nM	21
Total	14		1470