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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Neuropharmacology. 2015 Jul 16;99:735–749. doi: 10.1016/j.neuropharm.2015.06.017

Figure 1.

Figure 1

Experimental procedures for chronic intermittent ethanol vapor exposure (CIE), marble burying test (MBT), and slice electrophysiology recordings. A) Timeline for the CIE paradigm, in which mice were exposed to ethanol vapor for 16 hr/day for 4 consecutive days (constituting one cycle of CIE), followed by three days of abstinence, for four consecutive weeks. Mice underwent the MBT or were sacrificed for slice electrophysiology 48 hr after the last ethanol vapor exposure. B-C) Illustrations of brain subregions in which slice electrophysiology recordings were performed, including the: B) prelimbic cortex (PLC; in blue) and infralimbic cortex (ILC; in yellow) of the prefrontal cortex, C) dorsal BNST (dBNST; in blue) and ventral BNST (vBNST; in yellow), and D) lateral CeA (lCeA; in blue) and medial CeA (mCeA; in yellow).

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