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. Author manuscript; available in PMC: 2016 Apr 7.
Published in final edited form as: Oncogene. 2015 Jun 22;35(14):1750–1759. doi: 10.1038/onc.2015.239

Fig. 4. Depletion of FRS2α in PC3 cells in Matrigel plugs dampens recruitment of endothelial cells into the plugs in nude mice.

Fig. 4

A. Real-time RT-PCR analyses of Frs2α expression in PC3 cells infected with lentivirus carrying FRS2α or control shRNA. B. Gross morphology of the Matrigel plugs harvested from nude mice at two weeks after the implantation. C. Immunostaining of Matrigel plug sections with anti-CD31 or anti-VEGF-A antibodies. To-Pro3 (TP3) was used for nuclear counter staining. D. Real-time RT-PCR analyses of the RNA extracted from the Matrigel plugs containing control (n=6) or FRS2α-depleted (n=6) PC3 cells. mVEcad, mouse VE-cadherin; mCD31, mouse CD31; hVegf-a, human Vegf-a; shFRS2, FRS2α shRNA; Ctrl, control shRNA; *, P<0.05.