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. Author manuscript; available in PMC: 2016 Apr 7.
Published in final edited form as: Oncogene. 2015 Jun 22;35(14):1750–1759. doi: 10.1038/onc.2015.239

Fig. 6. Depletion of Frs2α in PCa cells reduces expression of Vegf-a.

Fig. 6

A. Real-time RT-PCR analyses of PC3 cells at 72 hours after treatment with the indicated siRNA at a final concentration of 25 or 100 mM. B. Luciferase reporter analyses of Vegf-a reporter activities in PC3 cells with or without depletion of expression of the indicated genes. C. Western blot analyses of VEGF-A expression in PC3 cells after reduction in expression of the indicated genes. D. Transwell cell culture analyses of HUVECs treated with conditioned medium of PC3 cells overexpressing cJUN or HIF1α. E. ChIP assays of Vegf-a promoter with anti-cJUN or anti-HIF1α antibodies. Panels a and c are the relative binding of cJUN or HIF1α to the V egf-A promoter. Data are mean ± sd derived from 3 replicate samples. Panels b and d are images of agarose gel electrophoresis showing single band of the PCR products. Ctrl, control siRNA; IP, immunoprecipitation; F/F, Frs2αflox; CN, Frs2αCN; *, P<0.05.