| Ingredient | % of Total | Volume (µl/10 ml) |
| 60x E3 Embryo Medium | 1.667 | 166.7 |
| Double deionized (ddi) H20 | 96.233 | 9623.3 |
| Alamar Blue | 1 | 100 |
| 400 mM NaOH | 1 | 100 |
| DMSO | 0.1 | 10 |
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