Primary antibodies	Dilution	Dilution buffer	Incubation	Secondary antibodies	Comments
Anti-Actin	1/10,000	PBS-T-BSA	15 min at RT	Anti-mouse	Use after SDS-PAGE  Diluted antibodyies can be reused several times if stored at 4 °C in the presence of 0.02 % Sodium Azide.
Anti-IRF3-P-Ser386	1/200	PBS-T-BSA	O/N at 4 °C	Anti-rabbit	Use after Native-PAGE.  It is not recommended to reuse the diluted antibody
Anti-IRF3-P-Ser396	1/10,000	PBS-T-BSA	O/N at 4 °C	Anti-rabbit	Use after SDS-PAGE.  It is not recommended to reuse the diluted antibody Anti-IRF3-P-396 is also available from Cell Signaling. Optimal dilution was defined as 1/1,000 for this antibody, but may vary between lots.
Anti-IRF3-P-Ser398	1/10,000	PBS-T-BSA	O/N at 4 °C	Anti-rabbit	Use after SDS-PAGE.  It is not recommended to reuse the diluted antibody
Anti-IRF3 full length	1/7,500	PBS-T-milk	3 hr at RT	Anti-rabbit	Use after SDS-PAGE.   Anti-IRF3 full length antibody can be used after native-PAGE, but it is less sensitive to detect the monomer. Diluted antibodies can be reused several times if stored at 4 °C in the presence of 0.02 % Sodium Azide.
Anti-IRF3-NES	0.5  μg/ml	PBS-T-milk	3 hr at RT	Anti-rabbit	Use after Native-PAGE. Diluted antibodyies can be reused several times if stored at 4 °C in the presence of 0.02 % Sodium Azide.
Anti-SeV	1/14,000	PBS-T-BSA	3 hr at RT	Anti-rabbit	Use after SDS-PAGE. Diluted antibodyies can be reused several times if stored at 4 °C in the presence of 0.02 % Sodium Azide.
NOTE: Dilution and buffer used for HRP-coupled secondary antibodies need to be optimized as it varies from one company to the other.