| Step 1 | Activation | 95 °C | 2 min | 1 cycle |
| Step 2 | Denaturation | 95 °C | 5 sec | 40 cycles |
| Annealing/Extension | 60 °C | 30 sec | ||
| Step 3 | Melt Curve (optional) | 65-95 °C (0.5 increments) | 5 sec/step |
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