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. 2016 Jan 30;(107):53518. doi: 10.3791/53518
Injected RNA Pool designation/selection Positive foxe3 expression
Library pools of 105 clones, 20 ng A 2/4
B* 4/28
C 4/44
Library pools of 104 clones, 20 ng 1 0/11
2 0/10
3 3/14
4 0/12
5 0/15
6 1/20
7 3/23
8 0/16
9 0/16
10* 5/20
Library pools of 5,000 clones, 20 ng 1 0/7
2* 5/16
3 1/7
4 0/7
5 0/7
Library pools of 400 clones, 20 ng 1 0/10
2 0/10
3 0/10
4 0/10
5* 8/26
6 0/11
7 0/10
8 0/10
9 0/10
10 0/8
Library pools of 70-200 colonies, 20 ng 1 0/8
2 0/10
3* 1/10
4* 1/10
5 0/10
6 0/9
7 0/10
8 0/10
Library pools of 20 colonies, 20 ng 1 0/10
2 0/10
3 0/10
4* 7/21
5 0/10
6 0/10
7 0/10
8 0/10
9 0/10
10 0/10
Library pools of 6 - 7 colonies, 20 ng K2-6, L1 0/10
L2-6, M7 0/10
M8-12, N7-8 0/10
K5-6, L1-4 1/10
L5-6, M7-10 0/10
M11-12, N7-8, K2-4 0/10
K2, K5, L2, L5, M8, M11 0/10
K3, K6, L3, L6, M9, M12 0/9
K4, L1, L4, M7, M10, N7, N8 1/9
Library RNAs, 20 ng K6 0/10
L1* 3/10
L3 0/10
L4 0/10
Library RNA, 20 ng L1 (ldb1) confirmation 50/179
* indicates selected pool