Table 1.
Effect of growth regulators on callus induction, shoot formation, and root induction of Brassica rapa ssp. pekinensis
| Growth regulators (mg l−1) | Callus per hypocotyl explants (%) | No. shoots from hypocotyl calli | Root induction (%) |
|---|---|---|---|
| BAP + NAA + AgNO3 | |||
| 1.0 + 3.0 + 4.0 | 35.25 ± 2.10d | ||
| 2.0 + 3.0 + 4.0 | 49.00 ± 2.76 cd | ||
| 3.0 + 3.0 + 4.0 | 65.00 ± 1.15b | ||
| 4.0 + 3.0 + 4.0 | 85.51 ± 1.12a | ||
| 5.0 + 3.0 + 4.0 | 51.42 ± 2.00c | ||
| BAP + NAA + AgNO3 | |||
| 4.0 + 3.0 + 4.0 | 1.52 ± 0.50e | ||
| 4.0 + 2.0 + 4.0 | 5.55 ± 0.75d | ||
| 4.0 + 1.0 + 4.0 | 9.21 ± 0.75c | ||
| 4.0 + 0.5 + 4.0 | 15.00 ± 0.55a | ||
| MS full strength | 59.00 ± 1.15b | ||
| MS half strength | 78.00 ± 1.00a | ||
| MS + IAA 1.0 | 49.52 ± 0.95c | ||
| MS + IBA 1.0 | 41.23 ± 1.01d | ||
Data represent mean values ± standard error (SE) of three replicates; each experiment was repeated thrice. Means with common letters are not significantly different at P ≤ 0.05 according to Duncan’s multiple range test (DMRT)