. 2016 Mar 7;6(1):88. doi: 10.1007/s13205-016-0402-1

Table 2.

Factors affecting transformation frequency of Brassica rapa ssp. pekinensis

Factors	No. of explants cultured	Transformation frequency (%)
Pre-culture days
0	30	0.0
1	30	7.0 ± 1.10^e
2	30	11.5 ± 2.00^bc
3	30	15.5 ± 1.00 ^a
4	30	10.5 ± 1.10^d
OD value
0.2	30	0.0
0.4	30	6.5 ± 1.00^ef
0.6	30	10.5 ± 1.50^d
0.8	30	15.5 ± 0.72 ^a
1.0	30	11.0 ± 1.25^c
Co-culture period
0	30	0.0
1	30	6.0 ± 1.00^f
2	30	12.2 ± 1.00^b
3	30	15.5 ± 1.12 ^a
4	30	10.0 ± 1.20^d
Acetosyringone (mg l⁻¹)
0	30	0.0
2	30	2.0 ± 1.00^f
4	30	12.2 ± 1.00^b
5	30	15.5 ± 0.50 ^a
6	30	10.0 ± 1.20^d

The best combinations of different factors (pre-culture, OD value, co-culture & acetosyringone) are shown in block letters. Data represent mean values ± standard error (SE) of three replicates; each experiment was repeated thrice. Means with common letters are not significantly different at P ≤ 0.05 according to Duncan’s multiple range test (DMRT)