Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 8;7:248. doi: 10.3389/fpls.2016.00248

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Berry, Tran, Dimos, Budziszek, Scavuzzo-Duggan and Roberts.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Flow cytometry analysis of P. patens protoplasts regenerated for 24 h and labeled with CBM or antibodies. (A) Scatter plot of protoplasts labeled with CBM3a and gated for roundness based on forward scatter (FSC) and side scatter (SSC). (B) Round protoplasts (population 1 above) gated for high and low chlorophyll autofluorescence. (C) Histogram of fluorescence intensities of population 1 showing peaks for subpopulations with high and low chlorophyll autofluorescence. (D) Mean fluorescence intensities (530/40) of regenerated protoplasts gated for roundness based on FSC and SSC and then for low vs. high chlorophyll autofluorescence. Differences in fluorescence intensity between high and low chlorophyll populations were significant (p = < 0.003) for all probes except LM18, LM19, and LM20, which were not significantly greater than the negative control.