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. 2016 Mar 8;7:248. doi: 10.3389/fpls.2016.00248

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Copyright © 2016 Berry, Tran, Dimos, Budziszek, Scavuzzo-Duggan and Roberts.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Physcomitrella patens rhizoids cultured on medium containing auxin (1 μM naphthalene acetic acid) for 2 weeks, fixed and labeled with antibodies or CBMs. Rhizoids were imaged with bright field (A,C,E,G,I,K,M,O) and fluorescence optics (B,D,F,H,J,L,N,P) after labeling with (A–D) anti-1,5-α-L-arabinan (LM6), (E,F) CBM3a for crystalline cellulose, (G,H) anti-callose (BS-400-2), which labels cross walls indicated by arrows, (I–L) anti-mannan (BS-400-4), which labels cell junctions indicated by arrows, (M,N) anti-HG (LM19), and (O,P) anti-arabinogalactan protein (LM2). Mature pigmented rhizoids are indicated with asterisk. Bar = 200 μm in (A) for (A,B). Bar = 100 μm in (C) for (C–P).