Table 1.
Summary of antibody and CBM labeling of Physcomitrella patens tissues.
| Probe | Epitope | CoMPP (Moller et al., 2007) | Protoplast | Protonema | Rhizoid | Gametophore |
|---|---|---|---|---|---|---|
| Glucans | ||||||
| CBM3a | Cellulose, crystalline | YES | YES | YES | YES | YES |
| CBM28 | Cellulose, non-crystalline | NT | YES | ND | ND | ND |
| BS 400-2 | Callose | YES | YES | YES | YES | YES |
| Hemicellulose | ||||||
| LM10 | Xylan, low-substituted | YES | ND | ND | ND | YES |
| LM15 | Xyloglucan, non-fucosylated | YES | YES | YES | ND | ND |
| BS 400-4 | Mannan and glucomannan | YES | YES | YES | YES | YES |
| Pectin | ||||||
| JIM5 | Homogalacturonan, +/− methyl-esterified | YES | NT | NT | NT | YES |
| LM18 | Homogalacturonan, +/− methyl-esterified | NT | ND | NT | NT | YES |
| LM19 | Homogalacturonan, unesterified | NT | ND | YES | ND | YES |
| JIM7 | Homogalacturonan, methyl-esterified only | YES | NT | NT | NT | YES |
| LM20 | Homogalacturonan, methyl-esterified only | NT | ND | NT | ND | ND |
| LM5 | 1,4-β-D-galactan | YES | YES | NT | ND | YES |
| LM6 | 1,5-α-L-arabinan | YES | YES | YES | YES | YES |
| Glycoproteins | ||||||
| LM2 | Arabinogalactan protein | YES | YES | YES | YES | YES |
| JIM13 | Arabinogalactan protein | YES | YES | NT | ND | YES |
ND, none detected; NT, not tested.