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. 2016 Mar 8;10:86. doi: 10.3389/fnins.2016.00086

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Copyright © 2016 Pisa, Alonso, Rábano and Carrasco.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of fungal proteins in corpora amylacea from PD patients. Tissue sections of patient PD1 were obtained from the following regions: pons (PN), mesencephalon (MSP), hypothalamus (HT), callosal body (CB), and caudate and lenticular nuclei (CLN) (upper panels). Different regions of the CNS from five additional PD patients (PD2-PD6) were also analyzed (lower panels). Sections were incubated with rabbit polyclonal antibodies against C. albicans and P. betae (green) and a monoclonal antibody against α-tubulin (red). Sections were mounted and examined by confocal microscopy after incubation with the corresponding secondary antibodies. Overlapping red and green pixels appear as orange/yellow. DAPI and scale bar as in Figure 1.