Table 1.

Molecular methods for characterization of Mycoplasma pneumoniae.

Characterization scheme	Method(s)	Reference(s)
P1 gene typing	PCR-RFLP	Cousin-Allery et al., 2000
	PCR-high-resolution melt (HRM)	Schwartz et al., 2009b
	NASBA	Ovyn et al., 1996
	Sequencing	Dumke et al., 2006
	Pyrosequencing	Spuesens et al., 2010
	MALDI-TOF MS	Xiao et al., 2014
	NA-SERS	Henderson et al., 2015
Multilocus Variable-Number Tandem-Repeat Analysis (MLVA)	MLVA	Degrange et al., 2009
	MLVA (nested PCR)	Dumke and Jacobs, 2011
Multilocus sequence typing (MLST)	MLST	Brown et al., 2015b
Single nucleotide polymorphism (SNP) genotyping	SNaPshot minisequencing assay	Touati et al., 2015
Macrolide susceptibility genotyping	Sequencing	Lucier et al., 1995
	Pyrosequencing	Spuesens et al., 2010; Chan et al., 2013
	PCR-melting curve analysis	Wolff et al., 2008; Chan et al., 2013
Whole genome sequencing (isolates)	Shotgun sequencing	Himmelreich et al., 1996
	High-throughput sequencing	Lluch-Senar et al., 2015; Xiao et al., 2015
	Single-molecule long-read sequencing	Lluch-Senar et al., 2013