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. 2016 Mar 8;7:251. doi: 10.3389/fmicb.2016.00251

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Copyright © 2016 Ding, Shiu, Chen, Chiang and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Testosterone degradation in Endozoicomonas. The gene order of key genes required for degradation (A) and their corresponding reactions (B) presented in Endozoicomonas species. Degrading capabilities of test bacteria were confirmed by supplying 500 μM of testosterone in culture medium and sampled daily in the previous culture. Test bacteria are: E. montiporae (C), E. elysicola (D), E. numazuensis (E), V. corallilyticus (F), and a blank control without inoculum (G). Abbreviations used in TLC analysis are: 1-DT, 1-dehydrotestosterone; AD, androst-4-ene-3,17-dione; ADD, androsta-1,4-diene-3,17-dione; S, standard mixture; SF, solvent front; T, testosterone. Numbers stand for the day of culture.