Table 4.

Glyoxylate cycle enzymes (ICL and MS) in selected marine invertebrates.

Type	Organism name	Data source	Number of BLAST hit
			ICL	MS
Coral	Acropora digitifera	Genome	1	2
	Acropora millepora	ESTs	1	2
	Montastrea faveolata	ESTs	3	1
	Porites astreoides	ESTs	2	2
Sea anemone	Aiptasia pallida	ESTs	1	2
	Anemonia viridis	ESTs	1	0
	Nematostella vectensis	Genome	1	2
Sea slug	Elysia chlorotica	SRA	0	0
Sponge	Amphimedon quennslandica	Genome	0	0
	Leucosolenia complicata	Genome	11	2
	Oscarella carmela	Genome	0	0
	Sycon ciliatum	Genome	2	0

1. ICL and MS sequences from Saccharomyces cerevisiae were used as representitive sequences (queries).

2. When searching in EST database, tblastn was used and thresholds were set: e-value < 1e-15; bitscore > 100.

3. When searching in SRA database, tblastn was used first. Reads may affiliate with query sequences were recovered and subjected into blastn search against to nr/nt database. In blastn search, reads shared high identities and coverages to bacterial subjects will be excluded.